Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.

Author: Dailkis Tajora
Country: Azerbaijan
Language: English (Spanish)
Genre: Personal Growth
Published (Last): 25 February 2007
Pages: 452
PDF File Size: 7.66 Mb
ePub File Size: 18.91 Mb
ISBN: 640-5-86662-517-3
Downloads: 23723
Price: Free* [*Free Regsitration Required]
Uploader: Tokinos

Still can’t find the full text of the article? Chapman and Hall ltd, Japan; The plant extract 0.

Antioxidant and free radical scavenging activity of Spondias pinnata.

The IC 50 value of the extract was higher than that of the reference compound. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. Dif- extract or standard trolox suppressed the absorbance of ferent concentrations 0. Singlet oxygen scavenging assay. Nitric oxide radical scavenging inhibition of the plant extract was Determination of total phenolic content Total phenolic content was determined using Folin-Ciocalteu FC reagent according to the method of Singleton and Rossi [ 23 ] with a slight modification.

Flavonoids are scavengers of superoxide anions. Free Radic Res Furthermore, the in vivo antioxidant activity of this At sites of inflammation, the oxidation of Cl- ions by the extract needs to be assessed prior to clinical use. A higher IC50 values Table 1 of the extract and standard in this absorbance of the reaction mixture indicated greater assay were Author information Article notes Copyright and License information Disclaimer.

Phytochemical analysisantimicrobial and antioxidant activities of different parts of Pleocaulus sessilis Nees Bremek Acanthaceae H. Manganese dioxide was buffer 20 mM, pH 7.


Reactive species in ischemic rat lung injury: References Publications referenced by this paper. Generation of excess ONOO – leads to oxidative damage and tissue injury [ 39 ].

Antioxidant and free radical scavenging activity of Spondias pinnata.

Santanu Biswas Bose Institute India. Sustained levels of production of this radical are directly toxic to tissues and contribute to the vascular collapse associated with septic shock, whereas chronic expression of nitric oxide radical is associated with various carcinomas and inflammatory conditions including juvenile diabetes, multiple zctivity, arthritis and ulcerative colitis [ 35 ].

The IC50 values were compared by paired t tests. Structural features of the acidic polysaccharide of Spondias pinnata gum exudates. Please review our privacy policy.

Antioxidant and free radical scavenging activity of Spondias pinnata – Semantic Scholar

Hydroxyl radicals are ffee major active oxygen species causing lipid peroxidation and enormous biological damage [ 31 ]. Quercetin was used as positive control. Chap- oxide synthase gene expression.

Yazdanparast R, Ardestani A. Total phenolic content was determined using Folin-Ciocalteu FC reagent according to the method of Singleton and Rossi [ 23 ] with a slight modification.

Total antioxidant activity of plant extract and trolox. These changes contribute to cancer, atherosclerosis, cardiovascular diseases, ageing and scwvenging diseases [ 12 ]. Sourav Mandal and Mr. The total phenolic content was Obstetrics and Gynecology Addition of the plant extract to this pre-formed radical cation reduced it to ABTS in a concentration-dependent manner.

The assay was carried out as grade methanol with 1 volume of 1 mM xylenol orange described by Aruoma and Halliwell [19] with minor and 2. FEBS Lett Amelioration of chronic ileitis by nitric oxide synthase inhibition.


The results suggest that the plant extract is a more potent scavenger of superoxide radical than the standard quercetin. Sourav Mandal and Mr.

Curcumin was used as a standard. The bark of the S.

Potassium persulfate K2S2O8ethyl- solution was equilibrated to 0. Biochem J The results indicate that S.

There was a problem providing the content you requested

Studies on products of browning reactions: The scavenging activity was evaluated by measur- H2SO4. At sites of inflammation, the oxidation of Cl – ions by the neutrophil enzyme myeloperoxidase results in the production of another harmful ROS, hypochlorous acid [ 41 ].

The data represent the percentage superoxide radical inhibition.

An acidic solution 0. However, the activities of antioxidants have been attrib- uted to various mechanisms such as prevention of chain initiation, decomposition of peroxides, reducing capacity and radical scavenging [44]. Biochem Biophys Scavenginng Commun Korycka-Dahl M, Richardson T: The present study proved that the extract studied has more potent nitric oxide scavenging activity than the standard curcumin.

Finally, the reaction for 20 min and the absorbance was measured against an mixture was diluted to 1 ml with water and the absorb- appropriate blank. Hydrogen peroxide, potassium hexacyanofer- plot for the sample was divided by the gradient of the plot rate, Folin-Ciocalteu reagent, sodium carbonate for trolox.