Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.
|Country:||Antigua & Barbuda|
|Published (Last):||11 March 2015|
|PDF File Size:||14.65 Mb|
|ePub File Size:||1.1 Mb|
|Price:||Free* [*Free Regsitration Required]|
The main differences of our protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.
Term Bank – bact – French English Dictionary
The general validity of the method was demonstrated by experiments on nitrification inhibitors in enrichment cultures. Recently developed gene-probes techniques work well for the identification of particular strains, but are not yet useful for the numeration. The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. Language Portal of Canada Access a collection of Canadian resources on all aspects of English and French, including quizzes.
Nitrifying cell numbers and size in the pure cultures were determined by epifluorescence with a microscope, after DAPI staining. Switch to classic view.
Continuous enrichment cultures of nitrifying bacteria were obtained from an inoculum coming from the Seine estuary freshwater section.
A collection of writing tools that cover the many facets of English and French grammar, style and usage. Biovolumes were estimated according to cell size and converted in biomasses according to a conversion factor determined experimentally with a carbon analyser. Writing tools A collection of writing tools that cover the many facets of English and French grammar, style and usage.
Glossaries and vocabularies Access Translation Bureau glossaries and vocabularies. To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria.
The determined optimal growth rate was 0. They consist in measuring differences of ammonium, nitrite and nitrate dynamics in an inhibited and control sample during time.
Maybe the correct one is
Autotfophe Studies and Analyses . The most frequently used enumeration methods of nitrifying bacteria are not very satisfactory. C incorporation rates are measured by the incubation of samples with 14C-bicarbonate, the samples being filtered on 0. By acting this way, inhibition of autotrophic C incorporation by nitrifying bacteria was also complete, while heterotrophic incorporation was unaffected.
These methods can be applied as long as the inhibitors are specific for nitrifying bacteria, and activities are high enough to allow the measurement of concentration variations during incubation times which are not too long.
This work presents a reassessment of the autotrophic nitrifying activity measurement by the 14C-bicarbonate incorporation method and its use to estimate the biomass of nitrifying bacteria. Access a collection of Canadian resources on all aspects of English and French, including quizzes. Different methods of measuring the nitrifying activity exist.
By regenerating oxidised forms of nitrogen nitratethe nitrification process plays an important role in the nitrogen cycle of aquatic environments. Bicarbonate was measured by acid titration in natural water samples, and with the evolution method for culture samples. Change the order of display of the official languages of Canada English first French first Option to display the non-official languages Spanish or Portuguese Neither Spanish Portuguese Display definitions, contexts, etc. Our conclusion is that the results presented in this paper allow the validation of the 14C-bicarbonate incorporation method with and without inhibitors to measure the nitrifying activity.
These experiments consisted in measuring the effect of different combinations of N-serve, ethanol the organic solvent of N-serve and chlorate, on N-oxidation rates and C incorporation rates on samples of the two nitrifying enrichment cultures ammonium- and nitrite-oxidising bacteria. However, the ethanolic solution of N-serve had an unwanted result on C incorporation.
In which subject field? The method can also be used by placing the sample in optimal temperature, pH, oxygen and ammonium conditions for nitrifying bacteria, to allow potential nitrifying activity measurements.
Several methods were used for our study: Download the article in PDF to read it. For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent.
Une réévaluation de la methode d’incorporation – Revue des sciences de l’eau – Érudit
Revue des sciences de l’eau 11, no. The language you choose must correspond to the language of the term you have entered. This factor is considered to be constant in the standard incubation conditions. In this case, carbon incorporation rates can be converted in ammonium oxidation rates with the use of the conversion factor 0. A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass.
Classical culture techniques most probable number and immunofluorescence techniques are known to greatly underestimate the numbers of active organisms. Bacteria which are able to multiply by using inorganic matter as the only source of carbon and nitrogen.
The organic solvent enhanced heterotrophic incorporation of C which totally masked out the autotrophic contribution of nitrifying bacteria. The first methods developed were based on the use of specific nitrification inhibitors: FAQ Frequently asked questions Display options.
Revue des sciences de l’eau11 2—